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. 2006 Jan;3(1):31-3.
doi: 10.1038/nmeth842.

Whole-genome genotyping with the single-base extension assay

Frank J Steemers  1 , Weihua Chang, Grace Lee, David L Barker, Richard Shen, Kevin L Gunderson
Affiliations

Whole-genome genotyping with the single-base extension assay

Frank J Steemers et al. Nat Methods. 2006 Jan.

Abstract

We describe an efficient, accurate and robust whole-genome genotyping (WGG) assay based on a two-color, single-base extension (SBE), single-nucleotide polymorphism (SNP)-scoring step. We report genotyping results for biallelic International HapMap quality control (QC) SNPs using a single probe per locus. We show scalability, throughput and accuracy of the system by resequencing homozygous loci from our 100k Human-1 Genotyping BeadChip.

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