doi: 10.1271/bbb.69.2459.
Production of completely flavinylated histamine dehydrogenase, unique covalently bound flavin, and iron-sulfur cluster-containing enzyme of nocardioides simplex in Escherichia coli, and its properties
Affiliations
- PMID: 16377910
- DOI: 10.1271/bbb.69.2459
Item in Clipboard
Production of completely flavinylated histamine dehydrogenase, unique covalently bound flavin, and iron-sulfur cluster-containing enzyme of nocardioides simplex in Escherichia coli, and its properties
Biosci Biotechnol Biochem.
2005 Dec.
Free article
Abstract
The hmd gene of histamine dehydrogenase from Nocardioides simplex was overexpressed in Escherichia coli, and the resulting enzyme was purified to homogeneity. The purified recombinant enzyme is almost identical with the native enzyme in view of molecular weight and specific activity, and is stoichiometrically assembled with the three cofactors 6-S-cysteinyl FMN, 4Fe-4S cluster, and ADP.
Similar articles
-
6-S-cysteinyl flavin mononucleotide-containing histamine dehydrogenase from Nocardioides simplex: molecular cloning, sequencing, overexpression, and characterization of redox centers of enzyme.Biochemistry. 2004 Aug 24;43(33):10800-8. doi: 10.1021/bi049061q. Biochemistry. 2004. PMID: 15311941
-
Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination.J Biotechnol. 2005 Sep 29;119(3):260-71. doi: 10.1016/j.jbiotec.2005.04.005. J Biotechnol. 2005. PMID: 15964650
-
Cloning and characterization of histamine dehydrogenase from Nocardioides simplex.Arch Biochem Biophys. 2005 Apr 1;436(1):8-22. doi: 10.1016/j.abb.2004.11.024. Arch Biochem Biophys. 2005. PMID: 15752704
-
Reactions of nitric oxide and oxygen with the regulator of fumarate and nitrate reduction, a global transcriptional regulator, during anaerobic growth of Escherichia coli.Methods Enzymol. 2008;437:191-209. doi: 10.1016/S0076-6879(07)37011-0. Methods Enzymol. 2008. PMID: 18433630 Review.
-
Current understanding of enzyme structure and function in bacterial two-component flavin-dependent desulfonases: Cleaving C-S bonds of organosulfur compounds.Arch Biochem Biophys. 2024 Aug;758:110048. doi: 10.1016/j.abb.2024.110048. Epub 2024 Jun 5. Arch Biochem Biophys. 2024. PMID: 38848996 Review.
Cited by
-
Crystallization and preliminary crystallographic analysis of histamine dehydrogenase from Natrinema gari BCC 24369.Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):942-5. doi: 10.1107/S2053230X14011327. Epub 2014 Jun 19. Acta Crystallogr F Struct Biol Commun. 2014. PMID: 25005094 Free PMC article.
-
Red Shift in the Absorption Spectrum of Phototropin LOV1 upon the Formation of a Semiquinone Radical: Reconstructing the Orbital Architecture.J Phys Chem B. 2024 May 9;128(18):4344-4353. doi: 10.1021/acs.jpcb.4c00397. Epub 2024 Apr 30. J Phys Chem B. 2024. PMID: 38688080 Free PMC article.
