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. 2006 Feb;24(2):185-7.
doi: 10.1038/nbt1177. Epub 2006 Jan 1.

Derivation of human embryonic stem cells in defined conditions

Tenneille E Ludwig  1 , Mark E Levenstein, Jeffrey M Jones, W Travis Berggren, Erika R Mitchen, Jennifer L Frane, Leann J Crandall, Christine A Daigh, Kevin R Conard, Marian S Piekarczyk, Rachel A Llanas, James A Thomson
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Derivation of human embryonic stem cells in defined conditions

Tenneille E Ludwig et al. Nat Biotechnol. 2006 Feb.

Abstract

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

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Comment in

  • The medium is the message.
    Moore H. Moore H. Nat Biotechnol. 2006 Feb;24(2):160-1. doi: 10.1038/nbt0206-160. Nat Biotechnol. 2006. PMID: 16465158 No abstract available.

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