Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Dec;25(8):1255-64.
doi: 10.1007/s10571-005-8502-1.

Fluoro Jade-B detection of dying cells in the SVZ and RMS of adult rats after bilateral olfactory bulbectomy

Affiliations

Fluoro Jade-B detection of dying cells in the SVZ and RMS of adult rats after bilateral olfactory bulbectomy

Barbora Mitrusková et al. Cell Mol Neurobiol. 2005 Dec.

Abstract

A novel fluorochrome, Fluoro-Jade B, was used to detect dying precursor cells in the subventricular zone (SVZ) and rostral migratory stream (RMS) of adult rats after bilateral olfactory bulbectomy and in control intact rats. The animals in experimental group were left to survive 3 days and from 3 till 16 months after surgical procedure. 1. In the control animals, Fluoro-Jade B positive cells were visible in the SVZ and within the whole extent of the RMS. The number of Fluoro-Jade B positive cells increased in the elbow in comparison to the rest parts of the RMS. 2. In the experimental animals surviving either 3 days or from 3 till 16 months after bilateral olfactory bulbectomy, Fluoro-Jade B positive cells displayed the similar pattern of distribution as in the control animals. However, some quantitative differences in the labeled cells number along the rostral migratory pathway appeared. 3. The average number of degenerating cells within the control SVZ and RMS was 26.24+/- 0.686. In bulbectomized animals, regardless of survival time, an insignificant increase of Fluoro-Jade B positive cells number occurred. We can conclude that dying of precursor cells is a physiological process running within the SVZ/RMS in both control and experimental animals. Moreover, this physiological process is not influenced by survival period after bilateral olfactory bulbectomy. Our results demonstrate Fluoro-Jade B as a useful marker of dying cells.

PubMed Disclaimer

References

    1. Altman, J. (1969). Autoradiographic and histological studies of postnatal neurogenesis. IV. Cell proliferation and migration in the anterior forebrain, with special reference to persisting neurogenesis in the olfactory bulb. J. Comp. Neurol.137:433–457. - PubMed
    1. Alvarez-Buylla, A., Garcia-Verdugo, J. M., Mateo, A. S., and Merchant-Larios, H. (1998). Primary neural precursors and intermitotic nuclear migration in the ventricular zone of adult canaries. J. Neurosci.18:1020–1037. - PMC - PubMed
    1. Ballok, D. A., Millward, J. M., and Sakic, B. (2003). Neurodegeneration in autoimmune MRL-lpr mice as revealed by Fluoro Jade B staining. Brain Res.964:200–210. - PubMed
    1. Brunjes, P. C., and Armstrong, A. M. (1996). Apoptosis in the rostral migratory stream of the developing rats. Brain Res. Dev. Brain Res. 92:219–222. - PubMed
    1. Burek, M. J., and Oppenheim, R. W. (1996). Programmed cell death in the developing nervous system. Brain Pathol.6:427–446. - PubMed

LinkOut - more resources