Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts
- PMID: 1639512
- PMCID: PMC257334
- DOI: 10.1128/iai.60.8.3442-3445.1992
Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts
Abstract
Polyclonal antibodies raised against Cryptosporidium parvum oocysts were found to cross-react with Eimeria spp. oocyst antigens in an indirect immunofluorescence assay, and sera from Eimeria spp.-infected lambs reacted with some antigens from sonicated C. parvum oocysts (between 29 to 30 and 66 to 69 kDa) by Western blot (immunoblot). No cross-reaction was observed with cystozoites of Toxoplasma and Sarcocystis spp. These results show the existence of epitopes common to C. parvum and various Eimeria spp.
Similar articles
-
Determination of immuno-cross-reactivity between Cryptosporidium parvum and Eimeria spp.Vet Parasitol. 1998 Mar 31;76(1-2):1-8. doi: 10.1016/s0304-4017(97)00225-2. Vet Parasitol. 1998. PMID: 9653985
-
Cross-reactivities with Cryptosporidium spp. by chicken monoclonal antibodies that recognize avian Eimeria spp.Vet Parasitol. 2005 Mar 10;128(1-2):47-57. doi: 10.1016/j.vetpar.2004.11.004. Epub 2004 Dec 15. Vet Parasitol. 2005. PMID: 15725532
-
Serological response to Cryptosporidium parvum in adult cattle from the Andean region of Colombia.Parasitol Res. 2001 Jun;87(6):500-4. doi: 10.1007/s004360100375. Parasitol Res. 2001. PMID: 11411953
-
Cryptosporidium parvum oocyst antigens recognized by sera from infected asymptomatic adult cattle.Vet Parasitol. 1995 Nov;60(1-2):17-25. doi: 10.1016/0304-4017(94)00770-d. Vet Parasitol. 1995. PMID: 8644454
-
Immunoassay for viable Cryptosporidium parvum oocysts in turbid environmental water samples.J Parasitol. 2001 Feb;87(1):203-10. doi: 10.1645/0022-3395(2001)087[0203:IFVCPO]2.0.CO;2. J Parasitol. 2001. PMID: 11227892
Cited by
-
Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt.J Parasit Dis. 2016 Sep;40(3):901-5. doi: 10.1007/s12639-014-0601-9. Epub 2014 Oct 30. J Parasit Dis. 2016. PMID: 27605806 Free PMC article.
-
Detection of infectious Cryptosporidium parvum oocysts in mussels (Mytilus galloprovincialis) and cockles (Cerastoderma edule).Appl Environ Microbiol. 2000 May;66(5):1866-70. doi: 10.1128/AEM.66.5.1866-1870.2000. Appl Environ Microbiol. 2000. PMID: 10788352 Free PMC article.
-
Obtaining hyperimmune anti-Cryptosporidium parvum ovine colostrum. A study of the humoral immune response in immunized sheep.Parasitol Res. 2006 Jan;98(2):119-29. doi: 10.1007/s00436-005-0031-9. Epub 2005 Nov 16. Parasitol Res. 2006. PMID: 16292678
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous