doi: 10.1016/0021-9673(92)80277-2.
Reversed-phase high-performance liquid chromatography-thermospray mass spectrometry of radiation-induced decomposition products of thymine and thymidine
Affiliations
- PMID: 1639896
- DOI: 10.1016/0021-9673(92)80277-2
Item in Clipboard
Reversed-phase high-performance liquid chromatography-thermospray mass spectrometry of radiation-induced decomposition products of thymine and thymidine
J Chromatogr.
.
Abstract
High-performance liquid chromatography-thermospray mass spectrometry was applied to the analysis of various radiation-induced decomposition products of thymidine including N-(2-deoxy-beta-D-erythro-pentofuranosyl)formamide and the various diastereomers of 5,6-dihydroxy-5,6-dihydrothymidine, 5-hydroxy-5,6-dihydrothymidine and 5,6-dihydrothymidine. This method combines high sensitivity and product resolution, rendering it particularly useful for monitoring the formation of radiation-induced base damage within DNA.
Similar articles
-
Characterization of radiation-induced products of thymidine 3'-monophosphate and thymidylyl (3'-->5') thymidine by high-performance liquid chromatography and laser-desorption Fourier-transform mass spectrometry.Radiat Res. 1994 Sep;139(3):271-9. Radiat Res. 1994. PMID: 8073109
-
Photooxidation of d(TpG) by riboflavin and methylene blue. Isolation and characterization of thymidylyl-(3',5')-2-amino-5-[(2-deoxy-beta-D- erythro-pentofuranosyl)amino]-4H-imidazol-4-one and its primary decomposition product thymidylyl-(3',5')-2,2-diamino-4-[(2-deoxy-beta-D- erythro-pentofuranosyl)amino]-5(2H)-oxazolone.Nucleic Acids Res. 1995 Oct 11;23(19):3954-61. doi: 10.1093/nar/23.19.3954. Nucleic Acids Res. 1995. PMID: 7479042 Free PMC article.
-
Comparison of radiolysis products of thymine and thymidine with e.s.r. results.Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Mar;27(3):211-22. doi: 10.1080/09553007514551511. Int J Radiat Biol Relat Stud Phys Chem Med. 1975. PMID: 166043
-
Identification of the cis-thymine glycol moiety in chemically oxidized and gamma-irradiated deoxyribonucleic acid by high-pressure liquid chromatography analysis.Biochemistry. 1981 Dec 22;20(26):7566-71. doi: 10.1021/bi00529a035. Biochemistry. 1981. PMID: 7326245
-
Critical practical aspects in the application of liquid chromatography-mass spectrometric studies for the characterization of impurities and degradation products.J Pharm Biomed Anal. 2014 Jan;87:191-217. doi: 10.1016/j.jpba.2013.04.027. Epub 2013 Apr 28. J Pharm Biomed Anal. 2014. PMID: 23706957 Review.
Cited by
-
Effects of heavy ions on nucleic acids: measurement of the damage.Radiat Environ Biophys. 1995 Mar;34(1):55-7. doi: 10.1007/BF01210547. Radiat Environ Biophys. 1995. PMID: 7604162 Review.