Grading follicular lymphoma on fine-needle aspiration specimens--a practical approach

Abstract

Fine-needle aspiration biopsy (FNA) is a useful tool for evaluating and staging follicular lymphoma (FL) without subjecting patients to multiple excisional biopsies. The relative lack of architecture in FNA is a limitation in grading FL. Recognizing the various cellular components in aspirates of FL, correlating with flow cytometry, using other ancillary tests, and preparing cell blocks can help overcome difficulties in the grading process. Understanding the clinical relevance of distinguishing the various grades of FL is important in determining whether an aspiration biopsy is adequate or whether additional sampling by surgical biopsy is indicated.