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. 2005 Oct;1(5):263-4.
doi: 10.1038/nchembio732. Epub 2005 Sep 18.

High-throughput assay for small molecules that modulate zebrafish embryonic heart rate

C Geoffrey Burns  1 , David J Milan, Eric J Grande, Wolfgang Rottbauer, Calum A MacRae, Mark C Fishman
Affiliations

High-throughput assay for small molecules that modulate zebrafish embryonic heart rate

C Geoffrey Burns et al. Nat Chem Biol. 2005 Oct.

Abstract

To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.

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