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. 2006 Jan;2(1):31-2.
doi: 10.1038/nchembio754. Epub 2005 Nov 27.

Direct transfer of extended groups from synthetic cofactors by DNA methyltransferases

Christian Dalhoff  1 , Grazvydas Lukinavicius, Saulius Klimasăuskas, Elmar Weinhold
Affiliations

Direct transfer of extended groups from synthetic cofactors by DNA methyltransferases

Christian Dalhoff et al. Nat Chem Biol. 2006 Jan.

Abstract

S-Adenosyl-L-methionine (AdoMet) is the major methyl donor for biological methylation reactions catalyzed by methyltransferases. We report the first chemical synthesis of AdoMet analogs with extended carbon chains replacing the methyl group and their evaluation as cofactors for all three classes of DNA methyltransferases. Extended groups containing a double or triple bond in the beta position to the sulfonium center were transferred onto DNA in a catalytic and sequence-specific manner, demonstrating a high utility of such synthetic cofactors for targeted functionalization of biopolymers.

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