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. 2005:404:95-107.
doi: 10.1016/S0076-6879(05)04010-3.

Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size

Joëlle Bigay  1 , Bruno Antonny
Affiliations

Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size

Joëlle Bigay et al. Methods Enzymol. 2005.

Abstract

The assembly-disassembly cycle of COPI and COPII coats is controlled by the GTPase cycle of the small G proteins Arf1 and Sar. We describe here two spectroscopic assays that enable real-time studies of some elementary steps of coat assembly and disassembly on artificial liposomes of defined composition and curvature. A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented.

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