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. 2005 Winter;5(4):410-3.
doi: 10.1089/vbz.2005.5.410.

West Nile virus in Southern Portugal, 2004

Aida Esteves  1 , A Paulo G Almeida, Rui Pedro Galão, Ricardo Parreira, João Piedade, João C Rodrigues, Carla A Sousa, M Teresa Novo
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West Nile virus in Southern Portugal, 2004

Aida Esteves et al. Vector Borne Zoonotic Dis. 2005 Winter.

Abstract

West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.

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