Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Oct 28;11(40):6305-11.
doi: 10.3748/wjg.v11.i40.6305.

Interleukin-17 levels in Helicobacter pylori-infected gastric mucosa and pathologic sequelae of colonization

Tomokazu Mizuno  1 , Takafumi Ando, Kazuo Nobata, Tomoyuki Tsuzuki, Osamu Maeda, Osamu Watanabe, Masaaki Minami, Kenji Ina, Kazuo Kusugami, Richard M Peek, Hidemi Goto
Affiliations

Interleukin-17 levels in Helicobacter pylori-infected gastric mucosa and pathologic sequelae of colonization

Tomokazu Mizuno et al. World J Gastroenterol. .

Abstract

Aim: To determine the role of interleukin (IL)-17 in gastric ulcerogenesis.

Methods: Thirty-six gastric ulcer (GU) patients and 29 non-ulcer (NU) patients were enrolled in this study. Mucosal biopsy samples were obtained from the gastric antrum and GU site during endoscopy. Samples were used in in situ stimulation for 48 h in the presence of 10 microg/mL phytohemagglutinin-P (PHA), histological examination, and Helicobacter pylori (H pylori) culture. IL-17 and IL-8 protein levels in culture supernatants were assayed by ELISA. IL-17 mRNA expression was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). H pylori cagA and vacA status was assessed by reverse hybridization using a line probe assay (LiPA). IL-8 levels in culture supernatants were assayed after AGS cells were co-cultured with H pylori strain 26,695 or recombinant human (rh) IL-17.

Results: All 36 GU patients and 15 of 29 NU patients were found to be H pylori-positive, while 14 NU patients were H pylori-negative. All 51 H pylori strains from both GU and NU patients were cagA- and vacAs1/m1-positive. Antral mucosal tissues from H pylori-positive patients contained significantly (H pylori-positive NU patients: median 467 pg/mg/protein, range 53-2,499; H pylori-negative NU patients: median 104 pg/mg/protein, range 16-312, P< 0.0005) higher levels of IL-17 than those from uninfected patients. IL-17 levels at the ulcer site were significantly (ulcer site: median 1,356 pg/mg/protein, range 121-1,3730; antrum: median 761 pg/mg/protein, range 24-7,620, P< 0.005) higher than those at distant sites in the antrum. Biopsies from H pylori-positive GU and NU patients showed IL-17 mRNA expression in all samples whereas those from the antrum of the H pylori-negative controls showed no detectable expression. A significant correlation was seen between IL-17 and IL-8 levels at each biopsy site (ulcer: r = 0.62, P< 0.0001; antrum: r = 0.61, P< 0.0001) in GU patients. RhIL-17 and H pylori strain 26,695 each stimulated IL-8 production from AGS cells.

Conclusion: IL-17 may play an important role in the inflammatory response to H pylori colonization, and may ultimately influence the outcome of H pylori-associated diseases that arise within the context of gastritis.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Levels of IL-17 (A) and IL-8 (B) secreted in organ cultures of biopsies from H pylori-positive (closed circle) and -negative (open circle) patients. The bar indicates the median value for each group. A: aP<0.0005 a vs b, bP<0.0001 a vs c, cP<0.0005 b vs c,dP<0.0001 a vs d, eP<0.0005 c vs d; B: aP<0.0005 a vs b, bP<0.0001 a vs c, cP<0.0005 a vs d, dP<0.0001 a vs d (NS:not significant).
Figure 2
Figure 2
Relationship between IL-17 and IL-8 levels in biopsy culture supernatants from antrum (A) and ulcer site (B). The bar indicates the median value for each group.
Figure 3
Figure 3
IL-17 (A) and IL-8 (B) levels in biopsy culture supernatants of GU patients according to ulcer stage.
Figure 4
Figure 4
Relationship between IL-17 (A and C) or IL-8 (B and D) levels and mononuclear cell infiltration or neutrophil infiltration at ulcer sites.
Figure 5
Figure 5
RT-PCR analysis of IL-17 and β-actin mRNA. M: 100-bp ladder marker; lane 1: negative (no cDNA sample); lane 2: positive control (PBMC stimulated by PHA for 3 h); lanes 3 and 4: antral biopsies from H pylori-negative NU patients with endoscopically normal mucosa; lanes 5 and 6: antral biopsies from H pylori-positive NU patients with gastritis; lanes 7, 9, and 11: antral biopsies from H pylori-positive GU patients; lanes 8, 10, and 12: mucosal biopsies from the ulcer site of H pylori-positive GU patients.
Figure 6
Figure 6
IL-8 levels in culture supernatants of AGS cells co-cultured with graded doses of rhIL-17, H pylori strain 26 695, or both.
See this image and copyright information in PMC

References

    1. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984;1:1311–1315. - PubMed
    1. Graham DY, Malaty HM, Evans DG, Evans DJ, Klein PD, Adam E. Epidemiology of Helicobacter pylori in an asymptomatic population in the United States. Effect of age, race, and socioeconomic status. Gastroenterology. 1991;100:1495–1501. - PubMed
    1. Blaser MJ. Hypothesis: the changing relationships of Helicobacter pylori and humans: implications for health and disease. J Infect Dis. 1999;179:1523–1530. - PubMed
    1. Crabtree JE, Taylor JD, Wyatt JI, Heatley RV, Shallcross TM, Tompkins DS, Rathbone BJ. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulceration, and gastric pathology. Lancet. 1991;338:332–335. - PubMed
    1. Dixon MF. Pathophysiology of Helicobacter pylori infection. Scand J Gastroenterol Suppl. 1994;201:7–10. - PubMed

MeSH terms