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. 2006 Jan;14(1):11-4.

[Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo]

[Article in Chinese]
Mei-xia Wang  1 , Qing-long Jin, Yu Pan, Feng Wang, Jun-qi Niu
Affiliations
  • PMID: 16420757

[Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo]

[Article in Chinese]
Mei-xia Wang et al. Zhonghua Gan Zang Bing Za Zhi. 2006 Jan.

Abstract

Objective: To determine the advantage of U1 small nuclear RNA as a ribozyme vector (U1-Rz) to inhibit HCV replication in vivo.

Methods: The 3rd stem-loop was substituted by HCV core specific ribozyme to construct an U1-Rz eucaryotic expression plasmid. Then it was co-transfected with pCMV/T7-NCRC Delta-luc into Huh7 cell line mediated by lipofectin. The cell lysis supernatant was subjected to Western blot and lumenometer to determine the luciferase levels.

Results: A U1 snRNA chimeric ribozyme was constructed successfully. Both Rz and U1-Rz inhibited luciferase expression in Huh7 by 48.64% and 87.46%, respectively.

Conclusion: Rz has more efficacy in cells when using U1 snRNA delivery system. U1 can be an efficient vector for HCV specific ribozyme.

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