doi: 10.1021/bi00677a003.
An investigation of the role of the copper in galactose oxidase
- PMID: 164209
- DOI: 10.1021/bi00677a003
Item in Clipboard
An investigation of the role of the copper in galactose oxidase
Biochemistry.
.
Abstract
Galactose oxidase is a metalloenzyme containing a single copper atom per molecule. The mechanism of action of galactose oxidase is studied in this paper by investigating substrate specificity and activation by peroxidase, and probing the copper site by electron spin resonance (ESR) spectroscopy. Line-shape simulation of ESR spectra are also reported and a comparison is made between observed and simulated spectra for galactose oxidase. A comparison is also reported for the enzyme from various commercial sources and enzyme isolated from a fungus in this laboratory. The results of this investigation suggest that the copper is in an environment of four in-plane nitrogens with axial symmetry.
Similar articles
-
[Fungal galactooxidase].Mikrobiol Zh (1978). 1979 Sep-Oct;41(5):560-9. Mikrobiol Zh (1978). 1979. PMID: 231187 Review. Russian. No abstract available.
-
Circular dichroism spectra of the copper enzyme, galactose oxidase, in the presence of its substrates and products.Biochemistry. 1974 Mar 12;13(6):1247-51. doi: 10.1021/bi00703a030. Biochemistry. 1974. PMID: 4360784 No abstract available.
-
Kinetic and magnetic resonance studies of substrate binding to galactose oxidase copper(II).J Inorg Biochem. 1981 Jun;14(3):223-35. doi: 10.1016/s0162-0134(00)80002-1. J Inorg Biochem. 1981. PMID: 6267193
-
Role of tryptophan in the spectral and catalytic properties of the copper enzyme, galactose oxidase.Biochemistry. 1977 Apr 19;16(8):1597-601. doi: 10.1021/bi00627a011. Biochemistry. 1977. PMID: 192267
-
The involvement of superoxide and trivalent copper in the galactose oxidase reaction.Adv Exp Med Biol. 1976;74:489-504. doi: 10.1007/978-1-4684-3270-1_42. Adv Exp Med Biol. 1976. PMID: 183480 Review. No abstract available.
Cited by
-
Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli.Protein Expr Purif. 2015 Apr;108:73-79. doi: 10.1016/j.pep.2014.12.010. Epub 2014 Dec 24. Protein Expr Purif. 2015. PMID: 25543085 Free PMC article.
-
Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion.iScience. 2023 Nov 17;26(12):108452. doi: 10.1016/j.isci.2023.108452. eCollection 2023 Dec 15. iScience. 2023. PMID: 38089572 Free PMC article.
-
A dual staining method for identifying mucins of different gastric epithelial mucous cells.Histochem J. 1991 Jan;23(1):22-8. doi: 10.1007/BF01886504. Histochem J. 1991. PMID: 1718927
-
Formation of Monofluorinated Radical Cofactor in Galactose Oxidase through Copper-Mediated C-F Bond Scission.J Am Chem Soc. 2020 Nov 4;142(44):18753-18757. doi: 10.1021/jacs.0c08992. Epub 2020 Oct 22. J Am Chem Soc. 2020. PMID: 33091303 Free PMC article.
-
Identification of Copper-Containing Oxidoreductases in the Secretomes of Three Colletotrichum Species with a Focus on Copper Radical Oxidases for the Biocatalytic Production of Fatty Aldehydes.Appl Environ Microbiol. 2021 Nov 24;87(24):e0152621. doi: 10.1128/AEM.01526-21. Epub 2021 Oct 6. Appl Environ Microbiol. 2021. PMID: 34613753 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Miscellaneous