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. 2005 Nov 28;11(44):7040-3.
doi: 10.3748/wjg.v11.i44.7040.

Cell survival curve for primary hepatic carcinoma cells and relationship between SF(2) of hepatic carcinoma cells and radiosensitivity

Zhi-Zhong Liu  1 , Wen-Ying Huang, Ju-Sheng Lin, Xiao-Sheng Li, Xiao Lan, Xiao-Kun Cai, Kuo-Huan Liang, Hai-Jun Zhou
Affiliations

Cell survival curve for primary hepatic carcinoma cells and relationship between SF(2) of hepatic carcinoma cells and radiosensitivity

Zhi-Zhong Liu et al. World J Gastroenterol. .

Abstract

Aim: To establish the cell survival curve for primary hepatic carcinoma cells and to study the relationship between SF(2) of primary hepatic carcinoma cells and radiosensitivity.

Methods: Hepatic carcinoma cells were cultured in vitro using 39 samples of hepatic carcinoma at stages II-IV. Twenty-nine samples were cultured successfully in the fifth generation cells. After these cells were radiated with different dosages, the cell survival ratio and SF(2) were calculated by clonogenic assay and SF(2) model respectively. The relationship between SF(2) and the clinical pathological feature was analyzed.

Results: Twenty-nine of thirty-nine samples were successfully cultured. After X-ray radiation of the fifth generation cells with 0, 2, 4, 6, 8 Gy, the cell survival rate was 41%, 36.5%, 31.0%, 26.8%, and 19%, respectively. There was a negative correlation between cell survival and irradiation dosage (r = -0.973, P<0.05). SF(2) ranged 0.28-0.78 and correlated with the clinical stage and pathological grade of hepatic carcinoma (P<0.05). There was a positive correlation between SF(2) and D0.5 (r = 0.773, P<0.05).

Conclusion: SF(2) correlates with the clinical stage and pathological grade of hepatic carcinoma and is a marker for predicting the radiosensitivity of hepatic carcinomas.

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Figures

Figure 1
Figure 1
Survival curve of hepatic carcinoma cells.
Figure 2
Figure 2
Relationship between survival rate of hepatic carcinoma cells and irradiation dosage.
Figure 3
Figure 3
Relationship between the SF2 (A) and D0.5 (B) in different clinical stages and pathological typing.
See this image and copyright information in PMC

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