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Review
. 2006 Mar 9;247(1-2):60-3.
doi: 10.1016/j.mce.2005.12.049. Epub 2006 Jan 27.

Estrogen mediated cross talk between the ovary and pituitary somatotrope. Pre-ovulatory support for reproductive activity

Gwen V Childs  1 , Mary Iruthayanathan, Noor Akhter, Brandy W Johnson
Affiliations
Review

Estrogen mediated cross talk between the ovary and pituitary somatotrope. Pre-ovulatory support for reproductive activity

Gwen V Childs et al. Mol Cell Endocrinol. .
No abstract available

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Figures

Plate 1
Plate 1
A. Dual labeling for Estrogen Receptor –Alpha isoform (ERα) in the nucleus (black) and growth hormone in the cytoplasm (orange-amber) of freshly dispersed cells from diestrous female rat. Most GH cells in the field express ERα. Cell C does not. B.Exposure to estradiol for 24 h (100 pM) stimulated expression of biotinylated Growth hormone releasing hormone (Bio-GHRH) binding sites, if given to estrous, metestrous, or diestrous female rats. C. Illustration of Bio-GHRH binding (gray-black) and labeling for growth hormone (orange-amber) in vehicle-treated diestrous female rats. D. Increased labeling for both Bio-GHRH (black) and GH (orange) is seen after 24 h in 100 pM estradiol. Insets show a higher magnification of dual labeled GH cells. Bar=15 μm
Plate 1
Plate 1
Figure 1.. Dual labeling for Estrogen Receptor –Alpha isoform (ERα) in the nucleus (black) and growth hormone in the cytoplasm (orange-amber) of freshly dispersed cells from diestrous female rat. Most GH cells in the field express ERα. Figure 2. Image analysis showed that 24 h in 0.1–1 nM estradiol produced an increase in average area of label for GH mRNA. Concentrations higher than 10 nM, however produced a decrease in labeling for the mRNA. Figure 3. shows this change by illustrating labeling for GH mRNA in cultures treated with vehicle (a), or 0.1 nM (b), 1 nM (c), or 250 nM estradiol. Bar=15 μm
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