Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2006 Mar 9;247(1-2):60-3.
doi: 10.1016/j.mce.2005.12.049. Epub 2006 Jan 27.

Estrogen mediated cross talk between the ovary and pituitary somatotrope. Pre-ovulatory support for reproductive activity

Gwen V Childs  1 , Mary Iruthayanathan, Noor Akhter, Brandy W Johnson
Affiliations
Review

Estrogen mediated cross talk between the ovary and pituitary somatotrope. Pre-ovulatory support for reproductive activity

Gwen V Childs et al. Mol Cell Endocrinol. .
No abstract available

PubMed Disclaimer

Figures

Plate 1
Plate 1
A. Dual labeling for Estrogen Receptor –Alpha isoform (ERα) in the nucleus (black) and growth hormone in the cytoplasm (orange-amber) of freshly dispersed cells from diestrous female rat. Most GH cells in the field express ERα. Cell C does not. B.Exposure to estradiol for 24 h (100 pM) stimulated expression of biotinylated Growth hormone releasing hormone (Bio-GHRH) binding sites, if given to estrous, metestrous, or diestrous female rats. C. Illustration of Bio-GHRH binding (gray-black) and labeling for growth hormone (orange-amber) in vehicle-treated diestrous female rats. D. Increased labeling for both Bio-GHRH (black) and GH (orange) is seen after 24 h in 100 pM estradiol. Insets show a higher magnification of dual labeled GH cells. Bar=15 μm
Plate 1
Plate 1
Figure 1.. Dual labeling for Estrogen Receptor –Alpha isoform (ERα) in the nucleus (black) and growth hormone in the cytoplasm (orange-amber) of freshly dispersed cells from diestrous female rat. Most GH cells in the field express ERα. Figure 2. Image analysis showed that 24 h in 0.1–1 nM estradiol produced an increase in average area of label for GH mRNA. Concentrations higher than 10 nM, however produced a decrease in labeling for the mRNA. Figure 3. shows this change by illustrating labeling for GH mRNA in cultures treated with vehicle (a), or 0.1 nM (b), 1 nM (c), or 250 nM estradiol. Bar=15 μm
See this image and copyright information in PMC

References

    1. Bethea CL. Estrogen action on growth hormone in pituitary cell cultures from adult and juvenile macaques. Endocrinology. 1991;129:2110–2118. - PubMed
    1. Chaidarun SS, Klibanski A, Alexander JM. Tumor-specific expression of alternatively spliced estrogen receptor messenger ribonucleic acid variants in human pituitary adenomas. J Clin Endocrinol Metab. 1997;82:1058–1065. - PubMed
    1. Chaidarun SS, Swearingen B, Alexander JM. Differential expression of estrogen receptor-β (ERβ) in human pituitary tumors: functional interactions with ERα and a tumor-specific splice variant. J Clin Endocrinol Metab. 1998;83:3308–3315. - PubMed
    1. Childs GV, Naor Z, Hazum E, Tibolt R, Westlund KM, Hancock MB. Localization of biotinylated gonadotropin releasing hormone on pituitary monolayer cells with avidin–biotin peroxidase complexes. J Histochem Cytochem. 1983a;31:1422–1425. - PubMed
    1. Childs GV, Naor Z, Hazum E, Tibolt R, Westlund KN, Hancock MB. Cytochemical characterization of pituitary target cells for biotinylated gonadotropin releasing hormone. Peptides. 1983b;4(4):549–555. - PubMed

Publication types

LinkOut - more resources