Review
doi: 10.1152/physiol.00045.2005.
Cryo-EM and single particles
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- PMID: 16443818
- DOI: 10.1152/physiol.00045.2005
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Review
Cryo-EM and single particles
Physiology (Bethesda).
2006 Feb.
Free article
Abstract
Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.
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