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Comparative Study
. 2005 Nov-Dec;41(10):343-8.
doi: 10.1007/s11626-005-0006-2.

Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium

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Comparative Study

Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium

Mainak DAS et al. In Vitro Cell Dev Biol Anim. 2005 Nov-Dec.

Abstract

In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology, the development of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth-promoting organosilane substrate coated on a glass surface and an empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3, brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the cultures were maintained for 4-6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional in vitro system.

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