Cloning and characterization of a fur homologue from Azospirillum brasilense Sp7
- PMID: 16450071
- DOI: 10.1007/s00284-005-0204-x
Cloning and characterization of a fur homologue from Azospirillum brasilense Sp7
Abstract
A homologue of the ferric uptake regulator gene, fur, was identified from a Azospirillum brasilense Sp7 genomic DNA clone. Experiments performed with transcriptional lacZ fusions demonstrated that the A. brasilense fur homologue regulated the expression of two fur regulated Escherichia coli genes: fiu (ferric iron uptake) and fhuF (ferric hydroxamate uptake). A differential regulation by the cognate Fur and the heterologous Fur homologue in response to the iron status of the growth medium was also observed.
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