Cloning and analysis of a genomic fragment of Dictyostelium discoideum hybridizing to an RNA specifically accumulated in spore cells
- PMID: 16453432
- PMCID: PMC553167
- DOI: 10.1002/j.1460-2075.1982.tb01301.x
Cloning and analysis of a genomic fragment of Dictyostelium discoideum hybridizing to an RNA specifically accumulated in spore cells
Abstract
A marker for Dictyostelium discoideum spore RNA differentiation has been isolated from a genomic DNA library by differential screening and a recombinant plasmid containing a genomic sequence complementary to spore specific RNA has been characterized. Only a small portion ( approximately 1 kb) of the 4.7-kb genomic insert is transcribed. The genomic organization of this spore specific gene shows a unique sequence flanked by reiterated sequences.
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