The nucleotide sequence of cowpea mosaic virus B RNA
- PMID: 16453487
- PMCID: PMC555442
- DOI: 10.1002/j.1460-2075.1983.tb01731.x
The nucleotide sequence of cowpea mosaic virus B RNA
Abstract
The complete sequence of the bottom component RNA (B RNA) of cowpea mosaic virus (CPMV) has been determined. Restriction enzyme fragments of double-stranded cDNA were cloned in M13 and the sequence of the inserts was determined by a combination of enzymatic and chemical sequencing techniques. Additional sequence information was obtained by primed synthesis on first strand cDNA. The complete sequence deduced is 5889 nucleotides long excluding the 3' poly(A), and contains an open reading frame sufficient to code for a polypeptide of mol. wt. 207 760. The coding region is flanked by a 5' leader sequence of 206 nucleotides and a 3' non-coding region of 82 residues which does not contain a polyadenylation signal.
Similar articles
-
Primary structure and gene organization of the middle-component RNA of cowpea mosaic virus.EMBO J. 1983;2(6):941-6. doi: 10.1002/j.1460-2075.1983.tb01525.x. EMBO J. 1983. PMID: 6641721 Free PMC article.
-
Complete nucleotide sequence of alfalfa mosaic virus RNA 2.Nucleic Acids Res. 1983 May 25;11(10):3019-25. doi: 10.1093/nar/11.10.3019. Nucleic Acids Res. 1983. PMID: 6304618 Free PMC article.
-
Complete nucleotide sequence of alfalfa mosaic virus RNA 1.Nucleic Acids Res. 1983 Mar 11;11(5):1253-65. doi: 10.1093/nar/11.5.1253. Nucleic Acids Res. 1983. PMID: 6298738 Free PMC article.
-
Cloned DNA copies of cowpea severe mosaic virus genomic RNAs: infectious transcripts and complete nucleotide sequence of RNA 1.Virology. 1992 Dec;191(2):607-18. doi: 10.1016/0042-6822(92)90236-i. Virology. 1992. PMID: 1448917
-
Nucleotide sequence of the capsid protein gene and 3' non-coding region of papaya mosaic virus RNA.J Gen Virol. 1988 Jan;69 ( Pt 1):219-26. doi: 10.1099/0022-1317-69-1-219. J Gen Virol. 1988. PMID: 3335832
Cited by
-
An NTP-binding motif is the most conserved sequence in a highly diverged monophyletic group of proteins involved in positive strand RNA viral replication.J Mol Evol. 1989 Mar;28(3):256-68. doi: 10.1007/BF02102483. J Mol Evol. 1989. PMID: 2522556 Free PMC article.
-
Cowpea mosaic virus VPg: sequencing of radiochemically modified protein allows mapping of the gene on B RNA.EMBO J. 1984 Jul;3(7):1629-34. doi: 10.1002/j.1460-2075.1984.tb02021.x. EMBO J. 1984. PMID: 16453534 Free PMC article.
-
In vitro expression of a full-length DNA copy of cowpea mosaic virus B RNA: identification of the B RNA encoded 24-kd protein as a viral protease.EMBO J. 1987 Mar;6(3):549-54. doi: 10.1002/j.1460-2075.1987.tb04789.x. EMBO J. 1987. PMID: 16453750 Free PMC article.
-
A Distinct Arabidopsis Latent Virus 1 Isolate Was Found in Wild Brassica hirta Plants and Bees, Suggesting the Potential Involvement of Pollinators in Virus Spread.Plants (Basel). 2024 Feb 28;13(5):671. doi: 10.3390/plants13050671. Plants (Basel). 2024. PMID: 38475517 Free PMC article.
-
Coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis.Nucleic Acids Res. 1989 Jun 26;17(12):4847-61. doi: 10.1093/nar/17.12.4847. Nucleic Acids Res. 1989. PMID: 2526320 Free PMC article.
References
LinkOut - more resources
Full Text Sources
Other Literature Sources