doi: 10.1002/j.1460-2075.1984.tb01814.x.
T-DNA of a crown gall tumor is organized in nucleosomes
Affiliations
- PMID: 16453504
- PMCID: PMC557352
- DOI: 10.1002/j.1460-2075.1984.tb01814.x
Item in Clipboard
T-DNA of a crown gall tumor is organized in nucleosomes
EMBO J.
1984 Feb.
Abstract
Nucleosomes were isolated from chromatin of suspension cultured cells of Nicotiana tabacum var. White Burley, which were either habituated or transformed by Agrobacterium tumefaciens, strain T37. Chromatin repeat length in both types of tissue was identical and can be estimated to be 195 +/- 10 bp. Using Southern transfer of nucleosomal DNA and hybridization with cloned nick-translated HindIII fragments of pTi C58 we show that the T-DNA originating from the Ti-plasmid of A. tumefaciens is organized in nucleosomes within the chromatin of crown gall tumor cells.
Similar articles
-
Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors.Proc Natl Acad Sci U S A. 1980 May;77(5):2666-70. doi: 10.1073/pnas.77.5.2666. Proc Natl Acad Sci U S A. 1980. PMID: 16592819 Free PMC article.
-
T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells.Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060-4. doi: 10.1073/pnas.77.7.4060. Proc Natl Acad Sci U S A. 1980. PMID: 16592850 Free PMC article.
-
Octopine synthase mRNA isolated from sunflower crown gall callus is homologous to the Ti plasmid of Agrobacterium tumefaciens.Proc Natl Acad Sci U S A. 1982 Jan;79(1):86-90. doi: 10.1073/pnas.79.1.86. Proc Natl Acad Sci U S A. 1982. PMID: 16593142 Free PMC article.
-
Historical account on gaining insights on the mechanism of crown gall tumorigenesis induced by Agrobacterium tumefaciens.Front Microbiol. 2014 Aug 7;5:340. doi: 10.3389/fmicb.2014.00340. eCollection 2014. Front Microbiol. 2014. PMID: 25147542 Free PMC article. Review.
-
Plant responses to Agrobacterium tumefaciens and crown gall development.Front Plant Sci. 2014 Apr 23;5:155. doi: 10.3389/fpls.2014.00155. eCollection 2014. Front Plant Sci. 2014. PMID: 24795740 Free PMC article. Review.
Cited by
-
Chromatin structure of integrated T-DNA in crown gall tumors.Plant Mol Biol. 1987 Mar;8(2):159-68. doi: 10.1007/BF00025327. Plant Mol Biol. 1987. PMID: 24301051
-
Insertion of the Mu1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure.Plant Mol Biol. 1986 Nov;6(6):441-54. doi: 10.1007/BF00027136. Plant Mol Biol. 1986. PMID: 24307421
-
Natural genetic engineering of plant cells: the molecular biology of crown gall and hairy root disease.World J Microbiol Biotechnol. 1996 Jul;12(4):327-51. doi: 10.1007/BF00340209. World J Microbiol Biotechnol. 1996. PMID: 24415308
References
LinkOut - more resources
Full Text Sources
