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. 1984 Dec 20;3(13):3039-41.
doi: 10.1002/j.1460-2075.1984.tb02254.x.

An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis

J P Hernalsteens  1 , L Thia-Toong, J Schell, M Van Montagu
Affiliations

An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis

J P Hernalsteens et al. EMBO J. .

Abstract

Cultured stem fragments from the monocotyledonous plant Asparagus officinalis infected by the oncogenic bacterium Agrobacterium tumefaciens developed tumorous proliferations. This tissue was propagated in vitro on hormone-free culture medium. The T-DNA-encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. The data demonstrate that stable T-DNA transfer as well as expression of T-DNA genes is possible in at least some monocotyledonous plants. This opens new possibilities for plant genetic engineering using the Ti plasmid as a gene vector.

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