Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Sep;5(9):2057-61.
doi: 10.1002/j.1460-2075.1986.tb04466.x.

cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products

R A Hooft van Huijsduijnen  1 , L C van Loon, J F Bol
Affiliations

cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products

R A Hooft van Huijsduijnen et al. EMBO J. 1986 Sep.

Abstract

A cDNA library was constructed to 10-15 S poly(A) RNA from tobacco mosaic virus (MV)-infected Samsun NN tobacco.By differential colony hybridization of 1400 transformants,32 clones were obtained corresponding to TMV-inducible tobacco mRNAs. These clones were subdivided into six clusters on the basis of cross-hybridization of the inserts. By Northern blot hybridization it was shown that three of the corresponding mRNAs were strongly induced by spraying tobacco plants with salicylic acid, whereas one mRNA was weakly induced by this treatment. All mRNAs were systemically induced in plants in which only the lower leaves were locally infected by TMV. Hybrid-selected translation was performed, using six clones representing one cluster each, followed by immunoprecipitation using an antiserum to purified pathogenesis-related (PR) proteins. Four clones yielded precipitable translation products. One of these clones represented a cluster of PR-1 clones, another clone encoded the thaumatin-like (TL) protein of tobacco which may correspond to PR-P or -Q.

PubMed Disclaimer

References

    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Gene. 1983 Nov;25(2-3):263-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253-7 - PubMed
    1. EMBO J. 1985 Nov;4(11):2745-9 - PubMed
    1. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 - PubMed

Publication types