Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants
- PMID: 16453764
- PMCID: PMC553917
- DOI: 10.1002/j.1460-2075.1987.tb02352.x
Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants
Abstract
A chimeric gene encoding the alfalfa mosaic virus (AlMV) coat protein was constructed and introduced into tobacco and tomato plants using Ti plasmid-derived plant transformation vectors. The progeny of the self-fertilized transgenic plants were significantly delayed in symptom development and in some cases completely escaped infection after inoculated with AlMV. The inoculated leaves of the transgenic plants had significantly reduced numbers of lesions and accumulated substantially lower amounts of coat protein due to virus replication than the control plants. These results show that high level expression of the chimeric viral coat protein gene confers protection against AlMV, which differs from other plant viruses in morphology, genome structure, gene expression strategy and early steps in viral replication. Based on our results with AlMV and those reported earlier for tobacco mosaic virus, it appears that genetically engineered cross-protection may be a general method for preventing viral disease in plants.
Similar articles
-
Expression of alfalfa mosaic virus coat protein in tobacco mosaic virus (TMV) deficient in the production of its native coat protein supports long-distance movement of a chimeric TMV.Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2549-53. doi: 10.1073/pnas.96.5.2549. Proc Natl Acad Sci U S A. 1999. PMID: 10051680 Free PMC article.
-
Lesions and virus accumulation in inoculated transgenic tobacco plants expressing the coat protein gene of tobacco mosaic virus.Virology. 1987 May;158(1):126-32. doi: 10.1016/0042-6822(87)90245-5. Virology. 1987. PMID: 18644562
-
Expression of alfalfa mosaic virus RNA 4 in transgenic plants confers virus resistance.EMBO J. 1987 Jul;6(7):1845-51. doi: 10.1002/j.1460-2075.1987.tb02442.x. EMBO J. 1987. PMID: 16453779 Free PMC article.
-
Methods for engineering resistance to plant viruses.Methods Mol Biol. 2007;354:183-95. doi: 10.1385/1-59259-966-4:183. Methods Mol Biol. 2007. PMID: 17172755 Review.
-
Genetically engineered protection against viruses in transgenic plants.Annu Rev Microbiol. 1993;47:739-63. doi: 10.1146/annurev.mi.47.100193.003515. Annu Rev Microbiol. 1993. PMID: 8257114 Review.
Cited by
-
Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus.Proc Natl Acad Sci U S A. 1990 Aug;87(16):6311-5. doi: 10.1073/pnas.87.16.6311. Proc Natl Acad Sci U S A. 1990. PMID: 2385595 Free PMC article.
-
Engineering virus resistance in agricultural crops.Plant Mol Biol. 1989 Sep;13(3):337-46. doi: 10.1007/BF00025322. Plant Mol Biol. 1989. PMID: 2485086 Review.
-
Agrobacterium mediated transfer of chlorsulfuron resistance to commercial flax cultivars.Plant Cell Rep. 1989 Dec;8(8):445-9. doi: 10.1007/BF00269045. Plant Cell Rep. 1989. PMID: 24233525
-
Plants that express a potyvirus proteinase gene are resistant to virus infection.Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6110-4. doi: 10.1073/pnas.90.13.6110. Proc Natl Acad Sci U S A. 1993. PMID: 8327491 Free PMC article.
-
Genetic engineering of plants for virus resistance.Arch Virol. 1990;115(1-2):1-21. doi: 10.1007/BF01310619. Arch Virol. 1990. PMID: 2248549 Review.
References
LinkOut - more resources
Full Text Sources
Other Literature Sources