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. 1991 May 20;219(2):217-30.
doi: 10.1016/0022-2836(91)90563-l.

Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter

M R Gartenberg  1 , D M Crothers
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Free article

Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter

M R Gartenberg et al. J Mol Biol. .
Free article

Abstract

Appropriately phased DNA bending sequences replacing the CAP binding site upstream from the lac promoter increase by roughly tenfold the rate of specific transcription initiation from a superhelical promoter template in vitro; promoter occlusion results from polymerase binding to the upstream (dA)n.(dT)n tracts, but this phenomenon is not responsible for the observed phase-dependent transcriptional activity. The rates of open complex formation at both P1 and P2 promoters respond in a similar phase-dependent way to the synthetic curved DNA sequences.

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