doi: 10.1128/mcb.11.6.3374-3378.1991.
Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene
Affiliations
- PMID: 1645450
- PMCID: PMC360192
- DOI: 10.1128/mcb.11.6.3374-3378.1991
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Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene
Mol Cell Biol.
1991 Jun.
Abstract
The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.
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