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. 1991 Jun;11(6):3374-8.
doi: 10.1128/mcb.11.6.3374-3378.1991.

Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene

S D Lupton  1 , L L Brunton, V A Kalberg, R W Overell
Affiliations

Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene

S D Lupton et al. Mol Cell Biol. 1991 Jun.

Abstract

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

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