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. 2006 Feb;44(2):334-9.
doi: 10.1128/JCM.44.2.334-339.2006.

Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species

Cristina Russo  1 , Enrico Tortoli, Donato Menichella
Affiliations

Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species

Cristina Russo et al. J Clin Microbiol. 2006 Feb.

Abstract

The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more frequently detected mycobacterial species and GenoType AS for less common species. The sensitivity and specificity were 97.9% and 92.4% for the CM kit and 99.3% and 99.4% for the AS kit. Our results show that the system may represent a useful tool to substantially enlarge the number of mycobacteria that can be reliably identified in clinical laboratories.

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Figures

FIG. 1.
FIG. 1.
Hybridization patterns produced in GenoType CM and AS with different mycobacterial species. 1, conjugate control; 2, universal probe; 3, genus Mycobacterium probe; 4 to 17, “species”-specific probes.
See this image and copyright information in PMC

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