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. 2006 Feb;44(2):607-11.
doi: 10.1128/JCM.44.2.607-611.2006.

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino  1 , Helena Barroso, Fátima Gonçalves, Sofia Marques Silva, Carlos Novo, Perpétua Gomes, Ricardo Camacho, Nuno Taveira
Affiliations

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino et al. J Clin Microbiol. 2006 Feb.

Abstract

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

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Figures

FIG.1.
FIG.1.
Patterns of reactivity of HIV plasma samples with rgp36 and rpC2-C3. Reactivity of HIV-1 and HIV-2 samples with rgp36 (A) and rpC2-C3 (B). (C) Low (LR) and high (HR) rpC2-C3 responders. S/CO, ODsample/ODcutoff ratio. Reactivity to rgp36 and rpC2-C3 is indicated by open (□) and black (▪) squares, respectively. The horizontal solid line represents the cutoff value; samples with S/CO values of ≥1 are considered reactive. The dotted line represents the lower S/CO value obtained with HIV-2 samples for rgp36. Student's t test was used to compare mean S/CO OD values obtained for both antigens.
FIG.1.
FIG.1.
Patterns of reactivity of HIV plasma samples with rgp36 and rpC2-C3. Reactivity of HIV-1 and HIV-2 samples with rgp36 (A) and rpC2-C3 (B). (C) Low (LR) and high (HR) rpC2-C3 responders. S/CO, ODsample/ODcutoff ratio. Reactivity to rgp36 and rpC2-C3 is indicated by open (□) and black (▪) squares, respectively. The horizontal solid line represents the cutoff value; samples with S/CO values of ≥1 are considered reactive. The dotted line represents the lower S/CO value obtained with HIV-2 samples for rgp36. Student's t test was used to compare mean S/CO OD values obtained for both antigens.
FIG.1.
FIG.1.
Patterns of reactivity of HIV plasma samples with rgp36 and rpC2-C3. Reactivity of HIV-1 and HIV-2 samples with rgp36 (A) and rpC2-C3 (B). (C) Low (LR) and high (HR) rpC2-C3 responders. S/CO, ODsample/ODcutoff ratio. Reactivity to rgp36 and rpC2-C3 is indicated by open (□) and black (▪) squares, respectively. The horizontal solid line represents the cutoff value; samples with S/CO values of ≥1 are considered reactive. The dotted line represents the lower S/CO value obtained with HIV-2 samples for rgp36. Student's t test was used to compare mean S/CO OD values obtained for both antigens.
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