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. 2006 Feb;44(2):607-11.
doi: 10.1128/JCM.44.2.607-611.2006.

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino  1 , Helena Barroso, Fátima Gonçalves, Sofia Marques Silva, Carlos Novo, Perpétua Gomes, Ricardo Camacho, Nuno Taveira
Affiliations

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino et al. J Clin Microbiol. 2006 Feb.

Abstract

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

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Figures

FIG.1.
FIG.1.
Patterns of reactivity of HIV plasma samples with rgp36 and rpC2-C3. Reactivity of HIV-1 and HIV-2 samples with rgp36 (A) and rpC2-C3 (B). (C) Low (LR) and high (HR) rpC2-C3 responders. S/CO, ODsample/ODcutoff ratio. Reactivity to rgp36 and rpC2-C3 is indicated by open (□) and black (▪) squares, respectively. The horizontal solid line represents the cutoff value; samples with S/CO values of ≥1 are considered reactive. The dotted line represents the lower S/CO value obtained with HIV-2 samples for rgp36. Student's t test was used to compare mean S/CO OD values obtained for both antigens.
FIG.1.
FIG.1.
Patterns of reactivity of HIV plasma samples with rgp36 and rpC2-C3. Reactivity of HIV-1 and HIV-2 samples with rgp36 (A) and rpC2-C3 (B). (C) Low (LR) and high (HR) rpC2-C3 responders. S/CO, ODsample/ODcutoff ratio. Reactivity to rgp36 and rpC2-C3 is indicated by open (□) and black (▪) squares, respectively. The horizontal solid line represents the cutoff value; samples with S/CO values of ≥1 are considered reactive. The dotted line represents the lower S/CO value obtained with HIV-2 samples for rgp36. Student's t test was used to compare mean S/CO OD values obtained for both antigens.
FIG.1.
FIG.1.
Patterns of reactivity of HIV plasma samples with rgp36 and rpC2-C3. Reactivity of HIV-1 and HIV-2 samples with rgp36 (A) and rpC2-C3 (B). (C) Low (LR) and high (HR) rpC2-C3 responders. S/CO, ODsample/ODcutoff ratio. Reactivity to rgp36 and rpC2-C3 is indicated by open (□) and black (▪) squares, respectively. The horizontal solid line represents the cutoff value; samples with S/CO values of ≥1 are considered reactive. The dotted line represents the lower S/CO value obtained with HIV-2 samples for rgp36. Student's t test was used to compare mean S/CO OD values obtained for both antigens.
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References

    1. Andersson, S., Z. da Silva, H. Norrgren, F. Dias, and G. Biberfeld. 1997. Field evaluation of alternative testing strategies for diagnosis and differentiation of HIV-1 and HIV-2 infections in an HIV-1 and HIV-2-prevalent area. AIDS 11:1815-1822. - PubMed
    1. Azevedo-Pereira, J. M., M. H. Lourenço, F. Barin, R. Cisterna, F. Denis, P. Moncharmont, R. Grillo, and M. O. Santos-Ferreira. 1994. Multicenter evaluation of a fully automated screening test, VIDAS HIV 1+ 2, for antibodies to human immunodeficiency virus types 1 and 2. J. Clin. Microbiol. 32:2559-2563. - PMC - PubMed
    1. Barroso, H., F. Araújo, M. H. Gomes, A. Mota-Miranda, and N. Taveira. 2004. Phylogenetic demonstration of two cases of perinatal human immunodeficiency virus type 2 infection diagnosed in adulthood. AIDS Res. Hum. Retrovir. 20:1373-1376. - PubMed
    1. Beelaert, G., G. Vercauteren, K. Fransen, M. Mangelschots, M. De Rooy, S. Garcia-Ribas, and G. van der Groen. 2002. Comparative evaluation of eight commercial enzyme linked immunosorbent assays and 14 simple assays for detection of antibodies to HIV. J. Virol. Methods 105:197-206. - PubMed
    1. Benitez, J., D. Palenzuela, J. Rivero, and J. V. Gavilondo. 1998. A recombinant protein based immunoassay for the combined detection of antibodies to HIV-1, HIV-2 and HTLV-I. J. Virol. Methods 70:85-91. - PubMed

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