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Multicenter Study
. 2006 Feb;44(2):619-21.
doi: 10.1128/JCM.44.2.619-621.2006.

High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

M Aires-de-Sousa  1 , K Boye, H de Lencastre, A Deplano, M C Enright, J Etienne, A Friedrich, D Harmsen, A Holmes, X W Huijsdens, A M Kearns, A Mellmann, H Meugnier, J K Rasheed, E Spalburg, B Strommenger, M J Struelens, F C Tenover, J Thomas, U Vogel, H Westh, J Xu, W Witte
Affiliations
Multicenter Study

High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

M Aires-de-Sousa et al. J Clin Microbiol. 2006 Feb.

Abstract

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

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