Use of molecular beacon probes for real-time PCR detection of Plasmodium falciparum and other plasmodium species in peripheral blood specimens
- PMID: 16455928
- PMCID: PMC1392706
- DOI: 10.1128/JCM.44.2.622-624.2006
Use of molecular beacon probes for real-time PCR detection of Plasmodium falciparum and other plasmodium species in peripheral blood specimens
Abstract
We describe the development and evaluation of a novel pair of real-time, fluorescence-based PCR assays using molecular beacon probes for rapid, sensitive, and specific detection and quantification of Plasmodium falciparum and other Plasmodium species organisms.
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References
-
- Church, D. L., A. Lichtenfeld, S. Elsayed, S. Kuhn, and D. B. Gregson. 2003. A regional centralized microbiology service in Calgary for the rapid diagnosis of malaria. Arch. Pathol. Lab. Med. 127:687-693. - PubMed
-
- Das, A., B. Holloway, W. E. Collins, V. P. Shama, S. K. Ghosh, S. Sinha, S. E. Hasnain, G. P. Talwar, and A. A. Lal. 1995. Species-specific 18S rRNA gene amplification for the detection of P. falciparum and P. vivax malaria parasites. Mol. Cell. Probes 9:161-165. - PubMed
-
- de Monbrison, F., C. Angei, A. Staal, K. Kaiser, and S. Picot. 2003. Simultaneous identification of the four human Plasmodium species and quantification of Plasmodium DNA load in human blood by real-time polymerase chain reaction. Trans. R. Soc. Trop. Med. Hyg. 97:387-390. - PubMed
-
- Elsayed, S., B. L. Chow, N. L. Hamilton, D. B. Gregson, J. D. Pitout, and D. L. Church. 2003. Development and validation of a molecular beacon probe-based real-time polymerase chain reaction assay for rapid detection of methicillin resistance in Staphylococcus aureus. Arch. Pathol. Lab. Med. 127:845-849. - PubMed
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