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. 1991 Mar;18(2-3):99-107.
doi: 10.1016/0168-1702(91)90011-j.

Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein

S Takase-Yoden  1 , T Kikuchi, S G Siddell, F Taguchi
Affiliations

Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein

S Takase-Yoden et al. Virus Res. 1991 Mar.

Abstract

A recombinant baculovirus system has been used to express the amino terminal half of the murine coronavirus (JHMV) peplomer glycoprotein in insect cells. The expressed polypeptide is glycosylated and is recognized by a set of monoclonal antibodies (mAbs) specific for JHMV S protein. Three of these mAbs have a very high neutralizing activity for JHMV but not for other MHV strains. These results indicate that JHMV-specific, major neutralizing epitopes reside in the amino terminal S1 subunit of the peplomer glycoprotein.

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