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. 1991 Jun:72 ( Pt 6):1309-15.
doi: 10.1099/0022-1317-72-6-1309.

High level transient expression of the murine coronavirus haemagglutinin-esterase

M Pfleiderer  1 , E Routledge, G Herrler, S G Siddell
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High level transient expression of the murine coronavirus haemagglutinin-esterase

M Pfleiderer et al. J Gen Virol. 1991 Jun.

Abstract

We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.

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