Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Sep;20(6):801-7.
doi: 10.1016/j.tiv.2005.12.003. Epub 2006 Feb 15.

p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Ssu-Ching Chen  1 , Ching-Hsein Chen, Chi-Liang Chern, Li-Sung Hsu, Ya-Chun Huang, King-Thom Chung, Soi-Moi Chye
Affiliations

p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Ssu-Ching Chen et al. Toxicol In Vitro. 2006 Sep.

Abstract

The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (DeltaPsim) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

PubMed Disclaimer

LinkOut - more resources