Quantitation of T-cell DNA in cutaneous lymphoid infiltrates

Abstract

A method is described that allows the quantitation of T-cell DNA within a tissue biopsy specimen. This method is based on the densitometric evaluation of the multiband pattern of TCR-gamma gene rearrangements detectable in Southern blot analyses of polyclonal T-cell DNA digested with HindIII. These rearrangements can be detected down to a limit of approximately 5% T-cell DNA. The information derived from this type of analysis can be used to roughly assess the total T-cell content of lesional tissue specimens expressed as a percentage of total tissue DNA, and can be correlated with the pattern of T-cell infiltration visualized by immunohistology. This assessment can be used also to determine the threshold, expressed as a percentage of total lesional T-cell DNA instead of total tissue DNA, required for the detection of a monoclonal T-cell population by Southern blot analysis. These quantitative relationships may prove useful for studying cutaneous T-cell lymphoma and associated precursor conditions.