doi: 10.1016/0014-5793(91)80689-z.
The Na+/H+ exchanger is phosphorylated in human platelets in response to activating agents
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- PMID: 1647984
- DOI: 10.1016/0014-5793(91)80689-z
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The Na+/H+ exchanger is phosphorylated in human platelets in response to activating agents
FEBS Lett.
.
Free article
Abstract
alpha-Thrombin, phorbol esters (PMA) and 1,2-diacylglycerol (DAG), three activators of the amiloride-sensitive Na+/H+ exchange in human platelets, rapidly increase the intracellular pH and the level of phosphorylation of the Na+/H+ exchange protein (NHE1). This stimulatory effect is suppressed by staurosporine, a potent kinase inhibitor, and increased by okadaic acid, a potent inhibitor of phosphatase 1 and 2A. The modulations of NHE1 phosphorylation by these factors correlate well with their effects on platelet pH. Thus, we conclude that in platelets (i) Na+/H+ exchange is mediated by NHE1, and (ii) platelet activating agents stimulate NHE1 via the modulation of the kinase/phosphatase equilibrium.
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