Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jul 1;88(13):5665-9.
doi: 10.1073/pnas.88.13.5665.

Regulation of a fos-lacZ fusion gene: a paradigm for quantitative analysis of stimulus-transcription coupling

K Schilling  1 , D Luk, J I Morgan, T Curran
Affiliations

Regulation of a fos-lacZ fusion gene: a paradigm for quantitative analysis of stimulus-transcription coupling

K Schilling et al. Proc Natl Acad Sci U S A. .

Abstract

Expression of the c-fos protooncogene is induced by a great variety of extracellular stimuli. A fos-lacZ fusion gene has been constructed that recapitulates this regulation. The fos-lacZ gene was introduced into B104 neuroblastoma cells for use in a quantitative assay for stimulus-transcription coupling. Both alpha- and beta-adrenergic agonists, dibutyryl cAMP, and phorbol ester induced beta-galactosidase activity in a dose-dependent manner. Thus, the interactions of receptors with agonists and antagonists, as well as intracellular second messenger-mediated signaling events, can be analyzed quantitatively. This approach represents a prototypic method for investigating stimulus-response coupling based upon gene expression.

PubMed Disclaimer

References

    1. J Biol Chem. 1965 Jun;240:2468-77 - PubMed
    1. Nature. 1974 May 17;249(454):224-7 - PubMed
    1. Annu Rev Neurosci. 1991;14:421-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7413-7 - PubMed
    1. J Biol Chem. 1988 Apr 15;263(11):4993-6 - PubMed

Publication types

MeSH terms

LinkOut - more resources