Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jul;173(14):4526-9.
doi: 10.1128/jb.173.14.4526-4529.1991.

IS231A from Bacillus thuringiensis is functional in Escherichia coli: transposition and insertion specificity

B Hallet  1 , R Rezsöhazy, J Delcour
Affiliations

IS231A from Bacillus thuringiensis is functional in Escherichia coli: transposition and insertion specificity

B Hallet et al. J Bacteriol. 1991 Jul.

Abstract

A kanamycin resistance gene was introduced within the insertion sequence IS231A from Bacillus thuringiensis, and transposition of the element was demonstrated in Escherichia coli. DNA sequencing at the target sites showed that IS231A transposition results in direct repeats of variable lengths (10, 11, and 12 bp). These target sequences resemble the terminal inverted repeats of the transposon Tn4430, which are the preferred natural insertion sites of IS231 in B. thuringiensis.

PubMed Disclaimer

References

    1. J Bacteriol. 1986 Aug;167(2):711-2 - PubMed
    1. EMBO J. 1985 Dec 30;4(13B):3895-9 - PubMed
    1. Plasmid. 1989 Jan;21(1):71-8 - PubMed
    1. Mol Gen Genet. 1989 Feb;215(3):416-24 - PubMed
    1. J Bacteriol. 1990 Dec;172(12):6689-96 - PubMed

Publication types

Substances

LinkOut - more resources