Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Apr 12;24(16):3224-31.
doi: 10.1016/j.vaccine.2006.01.042. Epub 2006 Feb 2.

Adherence of recombinant pneumococcal surface adhesin A (rPsaA)-coated particles to human nasopharyngeal epithelial cells for the evaluation of anti-PsaA functional antibodies

Affiliations

Adherence of recombinant pneumococcal surface adhesin A (rPsaA)-coated particles to human nasopharyngeal epithelial cells for the evaluation of anti-PsaA functional antibodies

Sandra Romero-Steiner et al. Vaccine. .

Abstract

Pneumococcal surface adhesin A (PsaA) is a pneumococcal vaccine candidate. In this study, we detect functional antibodies to PsaA by using carboxylate-modified fluospheres coated with either recombinant non-lipidated PsaA (rPsaA) or synthetic peptides with relevant epitopes of PsaA. Peptides P1-P3 were derived from phage display sequences; peptides P4-P7 were homologous to rPsaA. P1- and P4-coated fluospheres had similar adherence to Detroit 562 nasopharyngeal cells when compared to rPsaA-coated fluospheres. Homologous and heterologous competitive inhibitions with peptides in solution determined the specificity of the adherence. There was no significant difference (P=0.25) between the inhibition of adherence of rPsaA- and P4-coated fluospheres. This study indicates that P1 and P4 contain a functional epitope(s) for the adherence of PsaA to nasopharyngeal cells making them suitable targets for the measurement of functional antibodies to PsaA.

PubMed Disclaimer

Similar articles

Cited by

Publication types

LinkOut - more resources