Composition and production of thiol constituents induced by cadmium in the marine microalga Tetraselmis suecica

Abstract

Time course of intracellular levels of different thiols (phytochelatins, desglycyl-phytochelatins, glutathione, gamma-glutamylcysteine [gamma-Glu-Cys], and cysteine) were studied in the microalga Tetraselmis suecica exposed to different cadmium concentrations for 8 d. The cadmium concentrations assayed were 3, 7.9, 15, and 30 mg/L. Contents of thiol compounds synthesized by this microalga changed with cadmium concentration and with time of exposure. Cysteine concentrations increased significantly compared with those of gamma-Glu-Cys and glutathione in cultures containing 7.9, 15, and 30 mg/L. The increase in the amount of glutathione was significant only in cells exposed to the higher cadmium concentrations (15 and 30 mg/L). Nevertheless, in all the cadmium concentrations assayed, gamma-Glu-Cys levels were constant. A rapid increase in phytochelatins was observed with increased cadmium concentration during the first days of culture. The type of phytochelatins (number of subunits) also was dependent on the concentration of cadmium. The rate of (gamma-Glu-Cys)3-Gly accumulation was higher than those of other types of phytochelatins throughout the culture in cells exposed to 3, 15, and 30 mg/L. However, in cultures treated with 7.9 mg/L, (gamma-Glu-Cys)4-Gly increased substantially after 8 d of culture, exceeding the amount of (gamma-Glu-Cys)3-Gly. Cultures exposed to the higher cadmium concentrations (15 and 30 mg/L) showed a decrease in phytochelatins after 4 and 3 d of culture, respectively, whereas an increase in cysteine and glutathione occurred at the same time. A rapid decrease in phytochelatins also was measured when cells were placed into cadmium-free medium. This decrease is consistent with a degradation of the phytochelatins.