Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jul 11;19(13):3629-32.
doi: 10.1093/nar/19.13.3629.

MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase

M L Michaels  1 , L Pham, C Cruz, J H Miller
Affiliations
Free PMC article

MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase

M L Michaels et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have cloned chromosomal DNA bordering an insert that inactivates mutM. Sequencing of this clone has revealed that the insertion element is located between the promoter and structural gene for formamidopyrimidine-DNA glycosylase (Fapy-DNA glycosylase). An overproducing clone of Fapy-DNA glycosylase complements the original mutM strain that had been isolated after EMS mutagenesis. Thus, we conclude that MutM is actually Fapy-DNA glycosylase. mutM has previously been characterized as a mutator strain that leads specifically to G.C----T.A transversions. This in vivo characterization correlates well with the mutagenic potential of one of the lesions Fapy-DNA glycosylase removes, 8-oxo-7,8-dihydro-2'-deoxyguanine (8-OxodG).

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1954 Nov;40(11):1064-71 - PubMed
    1. Nucleic Acids Res. 1979 Aug 10;6(11):3673-84 - PubMed
    1. Biochemistry. 1991 Feb 5;30(5):1403-12 - PubMed
    1. Gene. 1990 Sep 1;93(1):1-7 - PubMed
    1. Nature. 1991 Jan 31;349(6308):431-4 - PubMed

Publication types

MeSH terms

Substances