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. 2006 Oct;103(1):62-6.
doi: 10.1016/j.ygyno.2006.01.033. Epub 2006 Feb 21.

Usefulness of combining testing for p16 protein and human papillomavirus (HPV) in cervical carcinoma screening

Tipaya Ekalaksananan  1 , Chamsai Pientong, Supannee Sriamporn, Bunkerd Kongyingyoes, Prasit Pengsa, Pilaiwan Kleebkaow, Onanong Kritpetcharat, D Max Parkin
Affiliations

Usefulness of combining testing for p16 protein and human papillomavirus (HPV) in cervical carcinoma screening

Tipaya Ekalaksananan et al. Gynecol Oncol. 2006 Oct.

Abstract

Objective: To evaluate the value of the combination of p16 and HPV detection in the screening for cervical cancer.

Methods: 186 patients with previous abnormal cervical lesion were studied. After colposcopic examination, two conventional Pap slides were prepared: the first was Papanicolaou-stained and examined by cytologist; the second was immunocytochemically stained for p16. Cervical cells were collected by brush using for HPV detection by Hybrid Capture II. Biopsy of any colposcopically abnormal lesions was performed.

Results: The 186 cervical samples were classified cytologically as normal (148), ASCUS (13), low-grade (11), high-grade (12) dysplasia and squamous cell carcinoma (2). P16 and HPV were found in all high-grade dysplasia and SCC, and in 64% and 27% of low-grade dysplasia, 62% and 0% of ASCUS and 7.4% and 3.4% of normal, respectively. 18 of p16-positive cases (11%) were HPV-negative, 14 of them in the ASCUS and normal group. Compared to histological results, all of the p16-positive cases of squamous metaplasia, CIN II/III and SCC were HR-HPV-positive. Therefore, the cases that were positive for both with normal cytology (5 cases) or low-grade dysplasia (3 cases) may comprise a high-risk group for neoplastic change.

Conclusion: The combination of p16 and HPV detection may be useful in cervical cancer screening to identify high-risk patients requiring early and proper management.

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