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. 1975 May 16;390(3):327-31.
doi: 10.1016/0005-2787(75)90353-6.

A transfer RNA-dependent protein synthesizing system from Ehrlich ascites extracts

O K Sharma, W K Roberts, D N Beezley, E Borek

A transfer RNA-dependent protein synthesizing system from Ehrlich ascites extracts

O K Sharma et al. Biochim Biophys Acta. .

Abstract

An homologous cell-free system from Ehrlich ascites cells was made tRNA dependent by passing both the ribosomes and the ammonium sulfate fraction through DEAE-cellulose columns in 0.30 M KCl. Protein synthesis was routinely stimulated 20-50 fold by the addition of 100 mug/ml of ascites cell tRNA in the presence of oviduct mRNA, ascites mRNA, or encephalomyocarditis RNA.

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