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. 1991 Aug;59(8):2579-85.
doi: 10.1128/iai.59.8.2579-2585.1991.

Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources

T Adam  1 , G S Gassmann, C Rasiah, U B Göbel
Affiliations

Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources

T Adam et al. Infect Immun. 1991 Aug.

Abstract

A total of 17 B. burgdorferi isolates from various sources were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, restriction enzyme analysis, Southern hybridization with probes complementary to unique regions of evolutionarily conserved genes (16S rRNA and fla), and direct sequencing of in vitro polymerase chain reaction-amplified fragments of the 16S rRNA gene. Three groups were distinguished on the basis of phenotypic and genotypic traits, the latter traced to the nucleotide sequence level.

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