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. 1991 Mar;25(2):141-4.
doi: 10.1016/0147-619x(91)90027-t.

Identification and DNA sequence of a new Bacteroides fragilis insertion sequence-like element

B A Rasmussen  1 , E Kovacs
Affiliations

Identification and DNA sequence of a new Bacteroides fragilis insertion sequence-like element

B A Rasmussen et al. Plasmid. 1991 Mar.

Abstract

A new Bacteroides fragilis insertion sequence (IS)-like element has been identified, cloned, and sequenced. The element is 1598 base pairs in length. It is flanked by a 15-base pair imperfect inverted repeat and contains a large open reading frame which could encode a 430 amino acid protein. There is an 8-base pair duplication of genomic DNA sequences at the site of integration. One copy of the IS-like element is integrated within the 5' upstream sequence of the metallo-beta-lactamase gene ccrA, cloned from B. fragilis TAL3636. The IS-like element is integrated 19 bp upstream of the predicted initiation codon and, therefore, probably provides the transcriptional start signals for the CcrA gene.

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