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. 2006 Mar;7(3):245-50.
doi: 10.1631/jzus.2006.B0245.

Effect of matrine and carvedilol on collagen and MMPs activity of hypertrophy myocardium induced by pressure overload

Ye-jing Zhang  1 , Mei-xiang Xiang, Jiang San, Gang Cheng, Shi-sheng Wang
Affiliations

Effect of matrine and carvedilol on collagen and MMPs activity of hypertrophy myocardium induced by pressure overload

Ye-jing Zhang et al. J Zhejiang Univ Sci B. 2006 Mar.

Abstract

Objective: To explore the effect and mechanism of matrine (Mt.) on myocardial interstitial fibrosis induced by pressure overload.

Methods: Pressure overloaded myocardial hypertrophy was produced by banding of aorta abdominalis in 67 male Sprague-Dawley rats weighing (200+/-15) g. The rats were assigned into one of the following groups: sham-operation control, operation control, operation group treated with matrine (15 mg/(kg.d)) and treated with carvedilol (Car.) (3.6 mg/(kg.d)) group. The rats were given drugs one day after operation. Five weeks after treatment, the left ventricular weight (LVW) was measured and the volume of myocardial cells was detected with Hematoxylin-Eosin (H-E) stain and Masson stain was used to assess the level of fibrosis of the myocardial matrix. Myocardial metalloproteinase activity was quantified with zymography, and survival rate was calculated.

Results: Survival rate significantly decreased (P<0.05), LVW/BW (body weight), MMP-2 (matrix metalloproteinase-2) activity (P<0.05), size of cardiomyocytes and interstitial fibrosis obviously increased in the operation group compared with sham control group. Mt. and Car. treatment can significantly increase survival rate (P<0.05), decrease LVW/BW (P<0.05) and MMP-2 activity (P<0.05), decrease size of cardiomyocytes and interstitial fibrosis compared with operation group. But there was difference compared with sham group.

Conclusion: Matrine was shown to be able to prevent cardiac remodelling of hypertrophy cardium induced by pressure overload including myocardial hypertrophy and fibrosis which may be associated with the decrease in MMP-2 activity of heart.

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Figures

Fig. 1
Fig. 1
The size of cardiomyocytes H-E stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 1
Fig. 1
The size of cardiomyocytes H-E stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 1
Fig. 1
The size of cardiomyocytes H-E stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 1
Fig. 1
The size of cardiomyocytes H-E stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 2
Fig. 2
Cardiac fibrosis with Masson stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 2
Fig. 2
Cardiac fibrosis with Masson stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 2
Fig. 2
Cardiac fibrosis with Masson stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 2
Fig. 2
Cardiac fibrosis with Masson stain×400 (a) Operation group; (b) Carvedilol treated group; (c) Matrine treated group; (d) Sham group
Fig. 3
Fig. 3
Cardiac hypertrophy among matrine group (T) operation group (C) and sham group (N)
Fig. 4
Fig. 4
(a) The differences of degrade bands of 2 groups: Mark, Sham-Oper., Mt.-Oper., Car.-Oper., Oper., Oper., Car.-Oper., Mt.-Oper., Sham-Oper.; (b) The differences of degrade bands of four groups, n=5, mean±SE Sham-Oper. (224.68±1.73) vs Oper. (244.66±0.47), Oper. vs Mt.-Oper. (228.37±2.02), Oper. vs Car-Oper. (236.51±0.52), Mt.-Oper. vs Car.-Oper.; *P<0.05, compared with Sham-Oper. and Oper.; #P<0.05, compared with Oper.and Oper.-treated; ΔP<0.05, compared with two-treated
Fig. 4
Fig. 4
(a) The differences of degrade bands of 2 groups: Mark, Sham-Oper., Mt.-Oper., Car.-Oper., Oper., Oper., Car.-Oper., Mt.-Oper., Sham-Oper.; (b) The differences of degrade bands of four groups, n=5, mean±SE Sham-Oper. (224.68±1.73) vs Oper. (244.66±0.47), Oper. vs Mt.-Oper. (228.37±2.02), Oper. vs Car-Oper. (236.51±0.52), Mt.-Oper. vs Car.-Oper.; *P<0.05, compared with Sham-Oper. and Oper.; #P<0.05, compared with Oper.and Oper.-treated; ΔP<0.05, compared with two-treated
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References

    1. Ai J, Gao HH, He SZ, Wang L, Luo DL, Yang BF. The influence of intracellular [Ca2+]i of ventricular myocytes of guinea pig treated with matrine. Acta Harbin Medical College. 2000;34(4):235–236. (in Chinese)
    1. Cohn JN. Structural basis for heart failure ventricular remodeling and its pharmacological inhibition. Circulation. 1995;91:2504–2507. - PubMed
    1. Doering CW, Jalil JE, Janicki JS, Pick R, Aghili S, Abrahams C, Weber KT. Collagen network remodelling and diastolic stiffness of the rat left ventricle with pressure overload hypertrophy. Cardiovasc Res. 1988;22:686–695. - PubMed
    1. Fung JW, Yu CM, Yip G, Chan S, Yandle TG, Richards AM, Nicholls MG, Sanderson JE. Effect of beta-blockade (carvedilol or metoprolol) on activation of the rennin-angiotensin-aldosterone system and natriuretic peptides in chronic heart failure. Am J Cardiol. 2003;92(4):406–410. doi: 10.1016/S0002-9149(03)00658-1. - DOI - PubMed
    1. Hu YH, Ouyang JP, Li YQ, Yang HL, Xu Y. The influence of cardiac fibroblast cycles and neucleo antigen expression of proliferate cells. Acta Wuhan University: Medical Edition. 2004;3:224–227. (in Chinese)

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