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Comment
. 2006 Mar 7;103(10):3500-1.
doi: 10.1073/pnas.0600551103. Epub 2006 Feb 27.

A milestone for hepatitis C virus research: a virus generated in cell culture is fully viable in vivo

Jens Bukh  1 , Robert H Purcell
Affiliations
Comment

A milestone for hepatitis C virus research: a virus generated in cell culture is fully viable in vivo

Jens Bukh et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

Conflict of interest statement: No conflicts declared.

Figures

Fig. 1.
Fig. 1.
Generation of J6/JFH1 viruses in cell culture and demonstration of their infectivity in vivo. Diagram showing study design and results in Lindenbach et al. (12). RNA transcripts from the chimeric genome were transfected into Huh-7.5 cells, and filtered supernatant was used to infect naïve Huh-7.5 cells. The immunostaining shown was performed with a mouse monoclonal NS5A antibody (1). Titrated virus from a virus stock was used to inoculate two chimpanzees i.v. and two SCID-uPA mice with a human liver graft i.p. Transfer of plasma from one of these viremic mice resulted in HCV infection in a third mouse. The specific infectivity (ratio of infectivity titer to RNA titer) was determined in naïve Huh-7 cells for chimpanzee and mouse serum containing the J6/JFH1 chimeric virus. TCID50, tissue culture 50% infective dose. Figure adapted, in part, from Lindenbach et al. (1).
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Comment on

References

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